2023 Challenger Hellcat Red Key Passion Down One Million

The length, width and height of 23 Dodge Hellcat SRT are 5027mm, 1923mm and 1461mm respectively. From the side photos, you can more intuitively understand its slender body design. The 20-inch granite crystal aluminum alloy wheel is matched with the 20-inch all-season performance tire, aiming to make the car have better dynamic performance. If a friend thinks that this design is not personalized enough, he can choose the side stripes of the challenger car body to further improve the recognition of the car. 24-hour exclusive hotline [150 2254 9999 (explained by Manager Sun of WeChat)]

  In terms of the appearance of the 2023 Dodge Challenger SRT Hellcat, the new car continues the American muscle car style with very tough and powerful challenger family models. At the same time, the new car has added a set of wide-body kit on the basis of the ordinary version, which is mainly aimed at widening its wheel eyebrows, and has increased by 3.5 inches (88.9mm) compared with the ordinary version, enabling the new car to use wider tires. In addition, the new car will also provide 13 different body colors, and provide a variety of body pull flowers for consumers to choose from.
24-hour exclusive hotline [150 2254 9999 (explained by Manager Sun of WeChat)]

  In the details of the 2023 Challenger SRT Hellcat, the middle net and the lower air intake of the new car are also painted in black, and there is a unique logo of SRT on the side of the middle net. In terms of wheels, SRT Super Stock adopts a set of 18x11J (18 inches in diameter and 11 inches in width) rims, which are the same as those of challenger SRT Demon, and is matched with the NT05R linear acceleration special tires with 315/40 R18 specifications, so that the car has three times the grip of SRT Hellcat models. In terms of brakes, the front wheel of the new car is equipped with an upgraded Brembo four-piston caliper and a 14.2-inch front brake disc.

24-hour exclusive hotline [150 2254 9999 (explained by Manager Sun of WeChat)]

  In terms of the power of 2023 Dodge Challenger SRT Hellcat, the new car is equipped with a 6.2-liter HEMI V8 supercharged engine. Compared with the challenger Hellcat Redeye model, its maximum power is increased from 807 horsepower to 818 horsepower, and the red line speed of the engine is increased from 6300rpm to 6400rpm. The transmission system is matched with a TorqueFlite 8HP90 8-speed automatic manual transmission. Dodge official said that the acceleration time of the new car from 0 to 96 km/h is 3.25 seconds, and it only takes 10.5 seconds to complete the linear acceleration of a quarter mile, but due to the tires of the new car, its top speed is limited to 168 mph (270 km/h).

 

24-hour exclusive hotline [150 2254 9999 (explained by Manager Sun of WeChat)]

Quick check! This kind of APP should be deleted quickly and be careful to participate in crime.

  Recently, several encrypted communication softwares, such as secret chat cat, Telegram, bat, secret communication and seagull, have frequently appeared in public notices issued by local police, which are accused of providing convenience for criminals to destroy criminal evidence. The police reminded that parents should check whether the above software is installed in their children’s mobile phones in time and delete it at the first time!

Quick check! This kind of APP should be deleted quickly and be careful to participate in crime.

  For example, WeChat official account, the official WeChat of Nanjing Public Security Bureau, announced on May 16th that the secret chat software, because of its strong privacy of encrypted communication, especially the function of "burn after reading", provided convenience for criminals to destroy criminal evidence, making the secret chat software a "gray area".

  The Nanjing Public Security Bureau said that nowadays, criminals have moved their servers from China to overseas, changed their communication tools from mass software to overseas secret chat software or domestic niche chat software, and changed their crime equipment from traditional "pseudo base stations" to "Modem pool", GOIP, Doka Bao, VoIP and so on. This kind of telecommunication network fraud crime committed by using new space and new equipment is highly technical, which increases the difficulty of investigating the case.

  Nanjing Public Security Bureau reminded that minors are not deeply involved in the world, and criminals cajole minors into providing personal information and other content through rhetoric on secret chat software, making them "accomplices" in illegal and criminal acts and even committing "helping the letter".

  The so-called "crime of helping the letter" is the crime of helping the information network criminal activities. The Criminal Law Amendment IX, which came into effect on November 1, 2015, added the crime of "helping information network criminal activities": providing technical support such as Internet access, server hosting, network storage, communication transmission, or providing assistance such as advertising promotion, payment and settlement, if the circumstances are serious, they shall be sentenced to fixed-term imprisonment of not more than three years or criminal detention, and shall also or shall only be fined.

  Compared with the crime of "helping the letter", online fraud is more common. The following fraud routines are aimed at student groups, please pay attention to parents!

  01

  Free skin "big trap"

  Nowadays, many children play games and pay special attention to "skin". Therefore, under the banner of "giving away skin for free", some scammers use the simple thinking of children and the weakness of not being able to communicate with parents to induce children to steal their parents’ mobile phones for transfer operations.

Quick check! This kind of APP should be deleted quickly and be careful to participate in crime.

  All kinds of initiatives to chat up, saying that there is free skin to collect, and then let you scan the code first and then thaw, are all scams!

  02

  Red envelope rebate "killing fish plate"

  Liars post red envelopes to return false information through a short video website, WeChat and other channels, and many primary school students will believe it and be pulled into the group.

  Liars first attract high profits as bait, and then cheat money with various excuses such as handling fees, transfer fees and red envelope fees. When the scam is discovered, the other party will be deleted or blacked out immediately.

Quick check! This kind of APP should be deleted quickly and be careful to participate in crime.

  03

  Fake "anti-addiction" to cheat money

  Nowadays, many children spend a lot of time at home, but they want to play games but are limited by the "anti-addiction system". They always want to find a way to crack it. Therefore, on the grounds of relieving the addiction of the game, the scammer deceived the minors to obtain their relatives’ WeChat or Alipay accounts and payment passwords through screen sharing, and then transferred the bank card property and defrauded the money.

Quick check! This kind of APP should be deleted quickly and be careful to participate in crime.

  04

  Fake stars send "fake benefits"

  Nowadays, many children like idolize, especially believing in idols. Liars generally work together, or one person plays multiple roles, and some pretend to be stars, with free benefits as bait; Some play brokers or fans, tricking students into bringing their parents’ mobile phones and cheating step by step.

  Common fraud methods include pretending to be a star, asking fans to help hit the list, and charging for entering the star fan base.

  How to effectively avoid cyber fraud?

  Always check the electronic products such as computers and mobile phones used by children in online classes. It is recommended to keep only the apps that must be used in class, and uninstall all other unrelated apps such as games, social networking and payment.

  Strengthen anti-fraud education for children. Don’t look at, ignore, or order calls, text messages, and links of unknown origin; Don’t pay attention to strangers who deliberately strike up a conversation on the internet, don’t covet petty gains, believe in winning prizes, earning extra money and other rhetoric; Do not listen, believe or transfer money for any reason.

  Parents should take care of their mobile phones, bank cards and other items. Don’t let your child know the payment password or change the password, and don’t set the payment APP as confidential payment, so as to avoid large-scale transfer and remittance after being deceived. At the same time, we should also pay attention to the balance of the child’s own WeChat account and Alipay account.

  Parents should put down their mobile phones when they have time, accompany their children to study, read and play more, and use a full life instead of playing mobile phones. Once you find that your child has been cheated, please immediately keep the evidence and call the police!

Source: Shanghai network rumors

The effect of measures to ensure supply and price stability continues to appear, and there are good conditions for market price stability.

  CCTV News:The State Council held a press conference on September 16th. At the press conference, Fu Linghui, spokesman of the National Bureau of Statistics, said that China’s market supply capacity is relatively strong, the effect of measures to ensure supply and stabilize prices will continue to appear, and there are good conditions for market price stability.

  In August, despite the influence of extreme weather such as high temperature and drought, the efforts to ensure supply and stabilize prices in the domestic market were gradually effective, the supply of domestic goods and services was generally sufficient, and the lagging influence of the downward trend of international oil prices was also emerging. In August, the consumer price changed from 0.5% in the previous month to 0.1% in the previous month, up 2.5% year-on-year, and the growth rate dropped by 0.2 percentage points from the previous month.

  From the ring comparison, the decline in consumer prices is due to the decline in the price increase of pork and fresh vegetables. The supply of live pigs was promoted in an orderly manner, and the slaughter of live pigs gradually recovered, which led to a decline in the price increase of pork. In August, the month-on-month increase in pork prices and vegetable prices dropped by 25.2 percentage points and 8.3 percentage points respectively.

The difference between domestic e-commerce and cross-border e-commerce

The difference between domestic e-commerce and cross-border e-commerce

When it comes to e-commerce, we all know that the familiar e-commerce platforms include Taobao, JD.COM and Pinduoduo, all of which are domestic e-commerce, but when it comes to cross-border e-commerce, many people may not understand.

Cross-border e-commerce is an international business activity in which transaction subjects belonging to different customs reach deals, make payment and settlement through e-commerce platforms, and deliver goods and complete transactions through cross-border logistics.

From the definition, we can see such three key words: transaction subjects in different customs, payment and settlement, and cross-border logistics. The difference between cross-border e-commerce and domestic e-commerce is also reflected here.

Take B2C export e-commerce as an example:

(1) Transaction subjects in different customs: Different customs means that compared with domestic e-commerce, cross-border e-commerce will face many complicated and changeable customs procedures, trade policies and other issues in import and export trade. For example: import and export tariffs, customs declaration, inspection and quarantine and so on.

(2) Payment and settlement: the cost is high, the payment takes a long time and there is exchange rate risk.

① Domestic e-commerce: The domestic e-commerce payment and settlement system is mature, and most of them use online banking payment and third-party payment methods such as Alipay and WeChat, which are convenient and fast, with low cost and short payment cycle (mostly within 15 days).

② Cross-border e-commerce: The payment system of cross-border e-commerce is relatively immature, especially for B2B large-scale trade. Most of them are paid by international payment tools such as PayPal, and the handling fee is as high as 3%-5% of the transaction amount, which has exchange rate risk and the payment period can be as long as 1-6 months. There is a great financial pressure on cross-border e-commerce sellers.

(3) Cross-border logistics: logistics has long time limit, high cost, low service level and high packet loss rate.

The difference between cross-border e-commerce and domestic e-commerce

1. Differences in business links

Cross-border e-commerce business links are more complicated. It needs to go through customs clearance, inspection and quarantine, foreign exchange settlement, export tax rebate, import tax collection and other links.

In the transportation of goods, cross-border e-commerce leaves the country by express delivery, and it takes longer for the goods to be sold to foreign consumers. Because of the long distance, the goods are easy to be damaged, and the express delivery capacity of various countries is relatively limited, and the growing number of parcels is also easy to cause trade friction. Domestic e-commerce takes place in China, and the goods are delivered to consumers by express delivery, with short journey, fast arrival speed and low probability of goods damage.

2. Differences of transaction subjects

The subjects of e-commerce transactions are generally in China, domestic enterprises to enterprises, domestic enterprises to individuals or domestic individuals to individuals. And the theme of the cross-border electronic commerce deal must be between customs.

It may be domestic enterprises to overseas enterprises, domestic enterprises to overseas individuals or domestic individuals to overseas individuals. Trading subjects spread all over the world and have different consumption habits, cultural psychology and living habits, which requires cross-border e-commerce to have a deeper understanding of the introduction of traffic from various countries, the promotion and marketing of various countries, the behavior of foreign consumers, and the construction of international brands, and the complexity is far beyond that of domestic e-commerce.

3. Differences in transaction risks

Domestic enterprises only have a weak awareness of property rights, and a large number of brand-free and low-quality goods and counterfeit goods flood the cross-border electronic commerce market, infringing on intellectual property rights and other phenomena occur from time to time.

In countries with perfect business environment and legal system, it is easy to cause intellectual property disputes. Domestic e-commerce behavior takes place in the same country, and both parties have a unified understanding of intellectual property rights such as trademarks and brands. There are fewer disputes caused by infringement, and disputes arise in time, which is shorter and more convenient.

4. Differences in applicable rules

Cross-border e-commerce needs to adapt to more detailed and complicated rules than domestic e-commerce. The first is the platform rules. In addition to domestic platforms, cross-border e-commerce operations may also conduct transactions on foreign cross-border e-commerce platforms, and each platform has different operating rules.

Cross-border e-commerce should be based on international general trade agreements and bilateral changeable trade agreements. Cross-border e-commerce should keep abreast of changes in the international trading system, rules, import and export controls and policies, and also have a deeper understanding and analysis of import and export forms. (Source: Logistics and Supply Chain Finance)

The above content belongs to the author’s personal opinion and does not represent Hugo’s position! This article is reproduced with the authorization of the original author, and it needs the authorization and consent of the original author.

The cruelest human body modification experiment in American history: twin babies were forced to change sex.

Speaking of transsexual surgery, it is no stranger to contemporary people.

With the development of science and technology and the improvement of human self-awareness, more and more transgender people use transsexual surgery to eliminate their physical and psychological pain caused by gender cognitive differences.

With the help of this kind of medical means, many of them have changed their growing experience of being misunderstood and discriminated against.

As the most recognized transgender in China,

Venus said: "I belong to the human part!

Gender is only my free choice. "

However, when transsexual surgery is not voluntary, will it bring the same result?

A warm and ordinary twin family, because of an accident, one of the boys was forced to undergo sexual organ transformation and become a girl when he was only 22 months old.

As a result, this "involuntary transsexual experiment" led by Dr. Harvard completely changed the fate of this family, leaving the two children in this family in a tangled gender cognition for the rest of their lives, and eventually they were broken and withered due to depression and mental breakdown. …

Horrible involuntary transsexual experiment

The story begins with a pair of Canadian twins, one named Bruce and the other named Brian, who were born on August 22nd, 1965.

Mothers and healthy twins

When they were just over half a year old, it was found that the foreskin was too long, which made it difficult to urinate, so the mother took her two babies to the hospital for circumcision.

Unfortunately, the medical technology was not developed at that time, and an accident happened in the middle of the operation. My brother Bruce’s genitals were burned completely by the electrocautery needle used in the operation.

Even now, reconstructing the lost male genitalia is still a high-risk operation for the newborn baby, so the doctor admits that Bruce’s injury is irreparable.

Mother is very sad and can only seek medical advice everywhere, hoping to save her children. Just when the husband and wife were on the verge of despair, they found Dr. john manny from the famous Johns Hopkins University in the United States.

John William Money,

The picture comes from Google.

This expert who focuses on research and psychology is often active in major TV programs, so he is quite famous. In the face of the help of the two couples, the doctor vowed that as long as the husband and wife are willing to help, he may help Bruce harvest a healthy life.

In john manny’s research field, he highly praised the "gender neutrality theory", believing that children have no concept of gender when they are born, and all their knowledge of gender comes from acquired education and environment. If children are "gender redistributed" when they are very young, and they are trained and educated, then they can get rid of their previous gender orientation.

In order to prove his theory, john manny did everything he could. At first, he tried to do a controlled experiment with bisexual people, and in order to get data to support his theory, he did not hesitate to falsify the experimental data.

However, people don’t pay for his experimental results, because bisexual people are vague about their own gender cognition, and with the influence of the day after tomorrow, it is indeed possible to have a bias towards a certain gender, so it is not universal.

The picture comes from Google.

Although john manny was frustrated by the result, he was helpless. Until he met the desperate Bruce couple.

Two same-sex twin babies, one maintaining the original sex and the other undergoing sex-change surgery, can form a perfect control group, and can also exclude the influence of some external uncertain factors, such as family factors and uterine environment. For john manny, this is simply a "godsend".

He quickly found the Bruce couple and persuaded them to let their two children participate in the experiment. He said that as long as he rebuilt his vagina, transformed Bruce into a girl and instilled the concept that he was a girl from an early age, he could become a woman!

This seems ridiculous now, but it has become the only lifeline for desperate parents. In order to make Bruce have a healthy and complete childhood, they accepted the doctor’s advice.

At this time, Bruce, who was only 22 months old, had sex replacement surgery, removed his testicles, changed from "he" to "she" and had a new name Brenda.

The picture comes from the Daily Mail.

After the transsexual operation, john manny injected Brenda with estrogen regularly.

At first, Brenda looked just like an ordinary girl, and her parents always took care of him as a "little girl" and instilled the idea that "you are a girl" in Brenda.

In this process, in order to stimulate Brenda’s female cognition, john manny adopted a series of operations that can be called "abnormal", such as showing Brenda naked photos of herself and her brother Brian, showing the completely different reproductive organs of men and women; Browse photos of women giving birth to baby girls and so on.

The picture comes from the Daily Mail.

In this way, until he was nine years old, john manny couldn’t wait to publish an article announcing that his experiment had been successful. However, he concealed the process of the experiment, and he didn’t mention anything about the adverse effects of the experiment and the psychological trauma caused to the two children.

After the article was published, it was supported and embraced by many people. Everyone went to Brenda’s home to interview, investigate and study, and the family’s life was forced to be placed in the attention of everyone.

Pictures come from the Internet.

Although everyone thinks Brenda is a girl, he has obvious "masculinity" from beginning to end. He likes to play with boys, solve problems by force, and prefer toys to dolls.

Moreover, since kindergarten, he has been mischievous and is the "king of problems" in the class. These personality characteristics show that Brenda is still a "boy" at heart, although her appearance has changed.

Pictures come from the Internet.

Because the original operation was not complete, with the growth of age, Brenda inevitably appeared some male characteristics, such as Adam’s apple and obvious muscles. The students around him gradually felt that he was different and crowded him out.

Brenda spent a long, lonely and self-abased youth in deep self-doubt.

Pictures come from the Internet.

After entering adolescence, Manny asked Brenda to have another artificial vagina operation, but Brenda had serious doubts about her gender cognition at this moment, so she was very resistant and unwilling to undergo the operation.

In the repeated struggle and self-doubt, the poor child suffered from severe depression and once tried to commit suicide. He didn’t know whether he was "him" or "her". Why are you so different from others? I don’t understand why everyone is asking him to be a "gentle and lovely" ordinary girl.

Watching his son fall into the dilemma of self-awareness and lose his happy smile bit by bit, his parents decided to stop the experiment and not force Brenda to inject estrogen or undergo sex modification surgery, and told Brenda the secret that had been hidden for 13 years.

He felt more relieved after being shocked, because his uniqueness finally found the answer.

Pictures come from the Internet.

Brenda gradually became cheerful and began to accept her identity as a "male". At the age of 16, he underwent penile reconstruction, changed his name to David Reimer.

At the age of 23, he got married and became the stepfather of three children.

Pictures come from the Internet.

However, after learning what happened to my brother, my brother Brian felt very unacceptable and his relationship with his brother deteriorated.

Growing up, he felt that Brenda had taken away all the attention from his parents and the outside world, which made him irritable. Later, he left this world sadly because he took too many antidepressants.

Pictures come from the Internet.

David didn’t finish his studies when he was a child, and his life was not smooth when he grew up. Later, he was cheated of money and was devastated. Looking at his broken family and his declining family, he chose to commit suicide.

At the age of 38, he shot himself. The bullet went through the temple, tore the brain stem fiercely and quickly, and took away his life. This devastated boy died in his car.

The distance between science and human nature

In fact, in history, so-called "scientists" like Manny who carried out terrorist experiments on human bodies under the guise of science abound, and they all brought serious psychological and physical trauma to the experimenters without exception.

The blind worship of science blurs the boundary between basic ethics and human nature.

Paul Feyerabend, an American philosopher of science, said in his book Against Methods: An Outline of Anarchism: "Science is conspicuous, grandstanding and rude. Only those who have decided to support a certain ideology, or those who have accepted science but have never examined its superiority and boundaries, will think that science is inherently superior."

The elite said that they had written the story of ——John B. Watson, an American behaviorist (click on the link: the parenting law that was once popular in the United States spread in China, and the experimental baby was devastated physically and mentally. Maybe your child is being cheated …). Watson conducted a series of gruesome experiments on the little boy "Albert" to prove his "crying immunity" education method, which was later called the most immoral experiment of psychology in the early twentieth century.

Pictures come from the Internet.

Watson hopes to apply what Pavlov learned from his research on dogs to people by observing Albert’s reaction. He constantly cultivates children’s fear of certain things and tests whether this fear can be transferred to other objects.

John B Watson wears a Santa mask to scare the baby.

In the experiment, little Albert was constantly frightened, and all he could give was to open his eyes in horror and cry … By the end of the experiment, the child’s expression was almost dull.

Pictures come from the Internet.

But like john manny, these "scientists" who focus on research never think that what they did led to the tragedy. Even though the experiments were widely criticized and criticized by the outside world, they themselves did not accept any punishment.

On September 25th, 1958, Waston died alone in Connecticut. Before his death, he burned all the unpublished scientific research results. Perhaps it was not until the end of his life that he realized his disregard and annihilation of human nature, and he began to question the truth he had always believed in through repeated destruction and destruction.

Pictures come from the Internet.

The evil in this world has many forms, even far beyond our imagination.

Time and time again, scientists have made selfish research, and one baby after another has been victimized by experiments. All this should not have happened. …

Science is rational, serious and even cold, but human beings are not. Whether it is extreme human experiments or genetic modification that touches the boundaries of life, if we abandon the most basic concern for human nature, it may lead to unpredictable tragic consequences.

Original title: "The most cruel human body transformation experiment in American history: Twin babies were forced to change sex, and an accident ruined two lives! 》

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The 70th anniversary of FAW Bus, the events between me and the bus, the feelings | "clothes and clothes", "love" bus for 26 years.

The 70th anniversary of FAW Bus, those things between me and the bus, those things.

In 2022

FAW Bus celebrates its 70th birthday.

After 70 years of hurricane and rain, I moved forward.

With the development of public transport in Guangzhou

It also witnessed the changes of Guangzhou city.

In the early stage, FAW Bus was launched.

"From generation to generation, you will live up to your youth."

Those things about me and the bus, those love works collection activities

Many citizens actively participate.

Share the friendship, love and affection on the bus with us.

Or a once-in-a-lifetime memory

Tell in words

FAW Bus has accompanied Guangzhou people through 70 years.

Today we share with you.

Old objects treasured by bus people.

"Clothes don’t give up", "Love" bus for 26 years

Guo Guiquan, former retired conductor of Route 69.

In 1996, I joined FAW Bus as a professional driver, and regarded the small bus as a big stage in my life. In more than 20 years of working, I have always been meticulous in vehicle maintenance, technical inspection of safety parts, ensuring that the vehicle is in good condition and providing high-quality and safe services for passengers. As a bus driver, I always go out early and come back late, and spend most of my time on the bus. However, I adhere to the purpose of taking the car as my home and taking the car as my pleasure, and not only have I accomplished the task assigned by the company well, but also won the honorary titles of "advanced individual" and "income expert".

"Collection"

work clothes

I cherish this relationship with the bus industry very much. During the years when I joined the bus service, I carefully treasured everything related to it, whether it was work clothes or a small badge. Whenever I open these "collections", I am as happy as a senior "bus fan", and the scenes of busy and hard work on the bus stage in recent years come into view again.

Guo Guiquan, former retired conductor of Route 69.

"Meticulous, serious and responsible" is my motto. I strive to be perfect and handle it in an orderly way whether I take care of my collection or face my daily life, and various honorary certificates issued by the company are the best affirmation of my work for more than 20 years. The persistence, concentration, persistence and preciseness I am pursuing have witnessed the enthusiasm of the older generation of bus people for their work and their enthusiasm for serving the masses.

Original title: "The 70th anniversary of FAW Bus, what happened between me and the bus |" Clothes and clothes ","Love "bus for 26 years"

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The availability of rhizosphere nutrients affects the role of saprophytic bacteria and mycorrhiza in litter decomposition and enzyme activity.

Compilation: Wei Ke Meng Suoya, Editor: Wei Ke Meng Suoya, Jiang Shunyao.

Wei Kemeng’s original micro-essay, welcome to forward and reprint, and the source of the reprint must be indicated in WeChat official account.

guide reading  

Professor Tian Xingjun from School of Life Sciences, Nanjing University and others published an article entitled "Synergy of saporphs with mycorhiza for little decomposition and hotspot formation dependencies on nutrient availability in the sphere" in Geoderma on December 27th, 2021. Plants can obtain and recycle nutrients from soil organic matter, and these dynamics depend on highly diverse rhizosphere microorganisms. Although the functions of many microbial groups have been determined, how different groups interact to influence biogeochemical processes is still poorly understood. In this study, the effects of the interaction between saprophytic bacteria and arbuscular mycorrhizal fungi (AMF) on the decomposition of alfalfa litter and the spatial distribution of enzyme activities in poor soil and nutrient-rich soil were studied by zymogram analysis.In poor soil, compared with single inoculation, co-inoculation of humic bacteria (Alcaligenes faecalis) or saprophytic fungi (Phanerochaete chrysosporium) with AMF(Rhizophagusi rregularis) can decompose litter faster and make the hot spot area of rhizosphere enzyme larger..The interaction between AMF and saprophytic bacteria is stronger than that with saprophytic fungi. The synergistic effect of saprophytic bacteria and AMF on litter decomposition and enzyme hot spot formation is weaker in eutrophic soil than in barren soil. On the contrary, in eutrophic soil, the co-inoculation of saprophytic fungi and AMF reduced litter decomposition.. PLS-PM analysis shows that,Faster litter decomposition and larger enzyme hot spots can promote plant growth.. Our results show that,There is an important synergistic relationship between these two microorganisms, which plays an important role in promoting plant growth in poor soil.. Among them,AMF can promote litter mineralization by increasing the biomass and enzyme activities of saprophytic bacteria (nitrate reductase, urease and cellulase).. At the same time, saprophytic bacteria increased the colonization rate of AMF and the hot spot of enzyme activity. These hot spots in turn expand the nutrient activation area around the roots-rhizosphere. In a word, the synergistic effect between saprophytic bacteria and AMF on litter decomposition and the formation of enzyme hot spots may be an important mechanism to promote plant adaptability under the condition of low soil nutrient availability.

sb/sth attracting attention   1. Arbuscular mycorrhizal fungi (AMF) can increase litter decomposition by stimulating saprophytic bacteria in malnourished soil. 2. inoculation with AMF can enlarge the hot spot area of rhizosphere enzyme activity by 13% ~ 400%. 3. In the soil with poor nutrients, AMF is more important for plant growth than in the soil with sufficient nutrients.    

Key words:Litter decomposition, rhizosphere process, nutrient cycle, plant-soil-microorganism interaction, soil zymogram

Paper ID

originName:Synergy of saprotrophs with mycorrhiza for litter decomposition and hotspot formation depends on nutrient availability in the rhizosphere

translatenameThe synergistic effect of saprophytic bacteria and mycorrhiza in litter decomposition and enzyme hot spot formation depends on the availability of rhizosphere nutrients.

journalGeoderma

IF:6.114

Publication time:2021.12.27

Communication workBy:Professor Tian Xingjun

Communication author unit:Nanjing University School of Life Sciences

DOI number:10.1016/j.geoderma.2021.115662

Experimental design

Medicago sativa L., a common host plant of AMF, was selected as the experimental plant and planted on two kinds of soil (nutrient-rich soil and barren soil). A total of 6 inoculation treatments (Control, not inoculated; Sapr-Bact, inoculated with saprophytic bacteria A. faecalis; alone; Sapr-Fungi, inoculated with saprophytic fungus p.chrysosporium alone; AMF, inoculated with R. irregularis; alone; Sapr-Bact/AMF, A. faecalis and R. irregularis were co-inoculated; Sapr-Fungi/AMF, co-inoculation of P. chrysosporium and R. irregularis). A total of 108 samples (6 inoculums × 2 nutritional levels × 3 sampling times × 3 replicates). After planting for 3 months, samples of plants, litter and soil were taken every month to determine the nutrient content. Microbial biomass, AME colonization, soil zymogram analysis and litter decomposition were determined.

1841640906339696

result

Table 1. Chemical properties of litter and soil

78651640906339858

Mean and standard deviation (n = 3)

5001640906339991

Figure 1. Design of experimental miniature basin. Nylon mesh (aperture 30 μm) separates the big basin from the small basin. AMF is added to the big pot, and saprophytic bacteria or fungi are added to the small pot. The top edge of the small pot is 10mm above the soil surface, which makes the pot easy to take out. The spatial distribution of enzyme activity on the surface of the inner hole of the big basin after removing the small basin was determined by zymogram.

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Fig. 2. The influence of litter decomposition rate (K value) (a), the mass loss of alfalfa litter (b), the correlation between substrate-induced respiration (SIR) and litter decomposition rate (c), and the SIR value (d) of inoculation amount at two soil nutrient levels after decomposition for 3 months. The value in (a) is the average of the influence of inoculation on the K value (n = 3) and the 95% confidence interval. Not exceeding the zero line indicates that the impact is significant. The values (b and d) are mean and standard deviation (n = 3 and 9). The letter indicates that there is a significant difference between the two kinds of soils under the same soil nutrient level, and the asterisk indicates that there is a significant difference between the two kinds of soils under the same microbial inoculation (p <0.05). (Control, not vaccinated; Sapr-Bact, inoculated with saprophytic bacteria A. faecalis; alone; Sapr-Fungi, inoculated with saprophytic fungus p.chrysosporium alone; AMF, inoculated with R. irregularis; alone; Sapr-Bact/AMF, A. faecalis and R. irregularis were co-inoculated; Sapr-Fungi/AMF, co-inoculation of P. chrysosporium and R. irregularis).

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Fig. 3. Principal component analysis (PCA) of enzyme activity (arrow) and decomposition rate (k) between different treatments. Characters are centered and standardized before sorting. (NR, nitrate reductase; URE, urease; ACP, acid phosphatase; ALP, alkaline phosphatase; BG, β -glucosidase; CEL, cellulase).

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Fig. 4. The zymogram of β -glucosidase activity, acid phosphatase activity and chitinase activity in poor soil three months after transplantation. The number in the upper left corner of each zymogram shows the percentage of hot spots (A). Color scale indicates enzyme activity (pmol mm-2h-1). Hot spot area percentage (b) of each inoculation point of the two soils after decomposition for 3 months. The values are mean and standard deviation (n = 3). The letter indicates that there is a significant difference between the two kinds of soils under the same soil nutrient level, and the asterisk indicates that there is a significant difference between the two kinds of soils under the same microbial inoculation (p <0.05).

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Fig. 5.PLS-PM shows the direct or indirect influence between soil nutrient level, AMF colonization rate, microbial biomass and microbial decomposition of litter, plant biomass and hot spots. The width of the arrow is proportional to the strength of the path coefficient. Blue and red arrows indicate negative and positive effects, respectively. The arrow indicates the significant effect (p <0.05). The numbers on the arrows represent significant standardized path coefficients. The R2 value represents the explained variance. Microbial functions include: nitrate reductase and urease activity; Plant biomass includes: aboveground biomass and root biomass; Enzyme activity hotspots include β -glucosidase activity, acid phosphatase activity and chitinase hotspot.

Supreme Leader: "Double Control Action" is a hard measure.

  During the "Thirteenth Five-Year Plan" period, a series of hard measures must be taken to promote the construction of ecological civilization and solve the problems of tight resource constraints, serious environmental pollution and ecosystem degradation, and only by doing real work can it be effective. General Secretary of the Supreme Leader pointed out in the explanation of the planning proposal of the Fifth Plenary Session: "It is a hard measure to implement the action of controlling the total amount and intensity of energy and water resources consumption and construction land. That is to say, it is necessary to control both the total amount and the energy consumption, water consumption and the intensity of construction land per unit of GDP. If this work is done well, it will not only save energy and water and soil resources, reduce pollutant emissions from the source, but also force the transformation of economic development mode and improve the green level of China’s economic development. " The Outline of the Thirteenth Five-Year Plan for National Economic and Social Development of the People’s Republic of China adopted at the Fourth Session of the 12th National People’s Congress well embodies this idea, and puts forward the action of "controlling the total amount and intensity of energy and water resources consumption and construction land". Please learn with "Learn China".

Pictured: The Fifth Plenary Session of the 18th Central Committee of the Communist Party of China (CPC) was held in Beijing from October 26 to 29, 2015. The General Secretary of the Central Committee and the Supreme Leader made an important speech.

First, dual control of energy consumption

  Energy is the source of power for modern economic growth and an important material basis for the sustainable development and security of national economy. With the rapid development of China’s economy and the continuous expansion of its economic aggregate, the process of industrialization and urbanization is also accelerating, energy consumption is increasing rapidly, and environmental degradation is also increasing. Under the constraint of energy and environment, to maintain the rapid and sustainable development of economy, we must build a safe, stable and environmentally friendly modern energy consumption system. At the meeting of the Central Leading Group for Finance and Economics held on June 14th, 2014, General Secretary of the Supreme Leader pointed out: "Resolutely control the total energy consumption, effectively implement the priority policy of energy conservation, make energy conservation run through the whole process of economic and social development and all fields, firmly adjust the industrial structure, attach great importance to energy conservation in urbanization, establish the concept of diligence and thrift, and accelerate the formation of an energy-saving society."

  Implementing total energy consumption control is an important strategy in China. In the past few decades, large-scale energy consumption has provided upward momentum for economic growth. During the Eleventh Five-Year Plan period, China’s total energy consumption increased from 2.36 billion tons of standard coal in 2005 to 3.25 billion tons in 2010, with an average annual growth rate of 6.6%. During the "Twelfth Five-Year Plan" period, although energy consumption was controlled, it still showed an overall growth trend. According to the statistical bulletin on national economic and social development in 2015 issued by the National Bureau of Statistics, the total energy consumption in 2015 was 4.3 billion tons of standard coal, up by 0.9% over the previous year. At present, China is changing from ensuring supply to controlling energy consumption, and open energy production and consumption will be restricted. In particular, high energy-consuming industries and surplus industries are subject to strong constraints on total energy consumption control, and other industries are subject to strong constraints according to advanced energy efficiency standards. From the perspective of national policy, the "Thirteenth Five-Year Plan" and medium-and long-term total control will continue. According to the National Energy Strategic Action Plan (2014-2020), by 2020, the total primary energy consumption will be controlled at about 4.8 billion tons of standard coal and the total coal consumption will be controlled at about 4.2 billion tons. The "Thirteenth Five-Year Plan" outlines: "The total energy consumption should be controlled within 5 billion tons of standard coal." Controlling the total energy consumption is actually the total consumption of coal and oil,Especially to reduce the proportion of coal consumption. In particular, strictly control the energy consumption of steel, cement, thermal power and other high energy-consuming industries and surplus industries, so that the total energy consumption of these industries will only decrease rather than increase.

  The index to measure the efficiency of regional energy utilization is mainly the intensity of energy consumption, that is, the energy consumption per unit of GDP. The higher the intensity of energy consumption, the lower the efficiency of energy utilization, otherwise, the higher the efficiency of energy utilization. The change of energy consumption intensity reflects the change of energy consumption structure on the one hand; On the other hand, it also reflects the change of industrial structure. Premier Li Keqiang pointed out in his 2016 government work report that during the Twelfth Five-Year Plan period, "the energy consumption per unit of GDP decreased by 18.2%, and the discharge of major pollutants decreased by more than 12%." The goal of the "Thirteenth Five-Year Plan" period is to reduce energy consumption per unit of GDP by 15%.

  Dual control of total energy consumption and energy intensity is an inevitable choice under the current economic restructuring, industrial upgrading and transformation, and air pollution control. We can’t wait for the peak of energy consumption to come naturally, so we have to take the initiative to regulate and break our wrists. From the perspective of energy management itself, to implement the goal of dual control, we must first change the demand-oriented energy production mode and turn to two-way management of demand side and supply side, improve energy utilization efficiency, ensure rational use of energy, encourage energy conservation, control excessive use of energy and limit extensive use of energy. In macroeconomic management, we should continue to increase the adjustment of industrial structure, speed up the development of modern service industry and cultivate and develop strategic emerging industries while transforming and upgrading traditional manufacturing industries. According to relevant research, if the secondary industry is reduced by one percentage point and the tertiary industry is increased by one percentage point, the energy consumption per unit GDP can be reduced by one percentage point. Obviously, the adjustment of industrial structure is the most effective measure to control the total energy and improve energy efficiency. The Outline of the Thirteenth Five-Year Plan puts forward: "Deepen the energy revolution, focus on promoting the transformation of energy production and utilization, optimize the energy supply structure, improve energy utilization efficiency, and build a clean, low-carbon, safe and efficient modern energy system."

Standardization of sign language and Braille in China ushered in a new milestone.

  A few days ago, the National Common Sign Language Glossary and the National Common Braille Program were officially released by the Ministry of Education, state language commission and China Disabled Persons’ Federation, and officially implemented on July 1, 2018. This means that sign language has "Mandarin" and Braille has "standard words".

  What is the significance of the publication of Thesaurus and Scheme? How was it developed? What are the characteristics? What problems should be paid attention to in the process of understanding and using? The reporter interviewed the heads of the two specification development teams.

  Further safeguard the language rights of the disabled.

  "This is an important achievement and a new milestone in the standardization of sign language and Braille in China, which has laid a solid foundation for future application and promotion, basic research, personnel training, discipline construction and social services." The person in charge of the development team of the two specifications said.

  In his view, the publication of the Thesaurus and the Program is the enrichment and supplement of the national common language, and it is the guarantee for the language rights of hearing-impaired and visual-impaired people to use sign language and Braille; It provides convenience for them to participate in social life equally and fully and share the development achievements of building a well-off society in an all-round way, which will further accelerate the standardization process of sign language and Braille and greatly promote the prosperity and development of culture and education for the disabled.

  The Thesaurus follows the principle of "adding new words, correcting mistakes and deleting old ones", and greatly supplements the number of commonly used words in sign language that reflect the development and changes of modernization, informationization and urbanization, so that it can basically meet the needs of hearing-impaired people in study, work and daily communication; Revise the actions in the original Chinese Sign Language that do not conform to the rules and characteristics of sign language expression for hearing disabled people; Delete obsolete words. After passing the final appraisal in 2015, it was piloted in 15 provincial deaf associations and 18 schools that enrolled deaf students, with thousands of participants.

  In 2010, the research on universal Braille was listed as an annual major project of the Twelfth Five-Year Scientific Research Plan of the State Language Committee and the China Disabled Persons’ Federation. The main task of the research is to fully absorb the advantages of existing Braille at home and abroad, adjust the existing rules of Braille transcription, realize comprehensive transcription, and reduce the length through simplification.

  Reflect the characteristics of the times and have stronger applicability.

  The person in charge introduced that the Thesaurus consists of two parts: one is to explain the scope of application, the definitions of some core terms used, the style and meaning of graphic symbols of sign language action lines, the basic principles of compilation and instructions for use; Secondly, according to the word order, the sign language widely used by the majority of hearing-impaired people in real life is included, which replaces many sign languages that correspond to Chinese characters in the past, greatly reducing the use of finger letters, paying attention to describing the changes of body movements and facial expressions during sign language expression, and focusing on embodying the language characteristics of sign language.

  "The Scheme follows the initial consonants, vowels, tones and punctuation marks of the current Braille, without changing, deleting or adding any symbols. It only perfects the current Braille phonetic transcription rules and standardizes the usage of tone symbols, which is the inheritance and development of the current Braille." The person in charge said that the "Program" shows the characteristics of convergence between the old and the new, accurate pronunciation, time-saving and party-saving, and is conducive to informationization.

  It is reported that accurate pronunciation is the primary goal of revising the rules of tone adjustment, and the tone of Braille, which is commonly used in the country, must be accurate. The perceptual characteristics of touch determine that Braille, as a tactile character, must be concise and try to improve the reading and writing speed of Braille as much as possible.

  Exploration of incorporating sign language norms into national language norms

  It is the first time since the reform and opening up 40 years ago that sign language norms have been included in the standard category of language and writing norms of the State Language Committee. The expression of sign language is as rich and flexible as audio language. What content is suitable for standardization and how to standardize it need further study. In this regard, the Lexicon has made a meaningful exploration.

  "At this stage, when using the Glossary, we need to grasp four groups of relationships, such as lingua franca and local sign language, life sign language and teaching sign language, sign language lexicology and use, and the Glossary and other countries’ common sign language reference books." The person in charge introduced that in terms of the use of common sign language and local sign language, the sign language actions presented in the Thesaurus are required to be used in official activities, school education, TV media, book publishing and other occasions or fields, as well as in the sign language level assessment held in the country in the future; Local sign language will still be used for a long time. In terms of life sign language and school sign language teaching, the Glossary specifically mentions "seeking common ground while reserving differences" and "beneficial to teaching". If there are regional differences or multiple ways of playing sign language vocabulary expressing the same thing and concept, the Glossary is juxtaposed, and there is no exclusivity. In terms of sign language lexicology and usage, the Thesaurus only shows the sign language actions of words, and the use of these words requires different sentence patterns. Sign language grammar has its own laws and characteristics and needs special study.

  "In addition, in terms of promotion, the China Disabled Persons’ Federation is working with the Ministry of Education, the State Language Committee and the State Administration of Radio, Film and Television to formulate a national universal sign language and national universal Braille promotion plan, focusing on special education institutions and public services, breaking through the difficulty of hearing and visual disabilities in society, and promoting national universal sign language and Braille step by step through backbone training, offering courses and improving relevant norms." The person in charge said. (Reporter Dong Luyi Long)

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