Consumer Rights Day takes you to know about invoice rights protection knowledge.

On the occasion of "March 15th International Consumer Rights Day", Chongqing tax authorities, in conjunction with the judicial and market supervision departments, organized personnel to go into business districts, plazas, markets and other places to distribute brochures, popularize consumer rights protection, invoice rights protection, tax law knowledge and other contents, guide business entities to standardize their operations, protect consumers’ legitimate rights and interests, and create a secure consumption environment.

Xiushan county tax officials came to the building materials market, gas stations, etc. to publicize invoice rights protection and tax law knowledge. Lai Tingying

Xiushan county tax officials came to the building materials market, gas stations, etc. to publicize invoice rights protection and tax law knowledge. Lai Tingying

"If the merchant refuses to issue invoices, consumers can provide clues to the tax authorities, which will order them to make corrections and impose a fine of less than 10,000 yuan. If there is illegal income, it will be confiscated." On March 12th, Zhongxian County Taxation Bureau organized a youth propaganda team of "Zhongba Car" to enter the local wanda plaza, and distributed "Invoice Use Risk Tips" to more than 100 settled businesses, informing them that when they collect money from business, they should issue invoices to consumers truthfully in time, and the legal consequences that enterprises will bear if they refuse to open or falsely issue invoices, so as to answer specific problems encountered by businesses in invoicing.

"We are still in the early stage of our business, and we don’t know much about taxation. Taxpayers come to the door to coach and invoice, reminding us of risks, which makes us more aware." Wang Yang, head of the Frodora children’s wear store in wanda plaza, Zhongxian County, said.

In the past few days, Chongqing tax authorities have focused on various small and medium-sized micro-business entities in the consumer market, organized personnel to explain the use norms and risk points of invoices, and provided one-on-one guidance on invoicing operations to guide them to operate in good faith according to law. For the vast number of consumers, through the distribution of brochures, broadcast promotional videos and other means, help consumers understand the tax knowledge closely related to their own rights and interests, encourage citizens to obtain invoices according to law, and actively resist fake invoices.

"Log in to the national VAT invoice inspection platform in State Taxation Administration of The People’s Republic of China and enter the invoice information to check the authenticity of the invoice in your hand." On March 15th, Yunyang County Taxation Bureau and the local market supervision bureau launched a consumer rights day publicity campaign. At the scene, tax officials explained to the public the way to check the authenticity of invoices, and sent hot question-and-answer manuals such as "No invoices for discounts", "No invoices for group purchase packages" and "No invoices for cross-month".

Chongqing tax authorities reminded that the current laws, regulations and related policies do not clearly stipulate the time limit for invoicing. The stipulations of merchants, such as not being able to issue invoices across months, are only unilateral acts and have no legal basis. Invoicing has nothing to do with whether it is discounted or not. Discounting is only a way for merchants to promote sales. Regardless of whether consumers buy goods at regular prices or consume discounted goods, as long as there is business behavior, sellers should issue invoices according to law.

At the publicity activities of Shapingba District International Consumer Festival, the tax officials in Shapingba District are explaining the invoice-related policies. Photo by Chen Saiya

At the publicity activities of Shapingba District International Consumer Festival, the tax officials in Shapingba District are explaining the invoice-related policies. Photo by Chen Saiya

On March 13th, the tax bureau of Wulong District organized eight staff members to distribute self-made comic brochures such as "Asking for invoices from merchants has these benefits" and "Guide to Consumer Invoicing" in the city square. "The merchant said that the service provided is not within the business scope of the business license and the ticket cannot be opened. Is it true?" In response to questions from the public, tax officials in Wulong District said that taxpayers should invoice according to the facts, even if they are beyond the business scope of the business license, except that the state has ordered them to ban sales and have industry access qualifications.

In the series of consumer rights day publicity activities, Chongqing tax authorities also set up a report account according to the reports and complaints of alleged infringement reflected by the people on the spot, and took corresponding measures to deal with, solve and feedback within a limited time to fully safeguard the legitimate rights and interests of consumers.

On the morning of March 15th, at the publicity activity of consumer rights protection held in Jinjie Square, West City, Baishiyi, Hi-tech Zone, the Taxation Bureau of Hi-tech Zone set up a reporting desk for tax violations. Ms. Zhang, a citizen, said, "Last week, I went to eat hot pot with my friends, and the store owner said that the checkout was discounted and I couldn’t issue an invoice."

"You tell me the consumption record, business information and contact information, and we will feedback the processing results to you as soon as possible after investigation and verification." The tax personnel recorded the report information reflected by Ms. Zhang on the spot and contacted the staff to handle the feedback in time.

On March 15th, in Shuiyuan Mall, Wuxi County, tax officials were explaining the contents of invoice rights protection to the public. Photo by Xiao Wei

On March 15th, in Shuiyuan Mall, Wuxi County, tax officials were explaining the contents of invoice rights protection to the public. Photo by Xiao Wei

According to the relevant person in charge of Chongqing Taxation Bureau, invoice disputes account for a large proportion in daily tax-related reporting cases. As long as there is consumption behavior, consumers have the right to ask the payee to issue invoices, regardless of the amount and method of consumption. The payee’s refusal to issue invoices or false invoices for any reason violates the Measures for the Administration of Invoices in People’s Republic of China (PRC).

If consumers encounter merchants who refuse to issue invoices for any reason or have doubts about the invoices they have received, they can call the 12366 tax payment service hotline or log on to the official website of Chongqing Taxation Bureau, and click "Report on Tax Violations" in the "Interactive Communication" column to report online. (Tang Jinrong)

Source: Chongqing Municipal Taxation Bureau

■ Extended reading

State Taxation Administration of The People’s Republic of China National VAT Invoice Inspection Platform: https://inv-veri.chinatax.gov.cn

Chinese Academy of Sciences patent data integrated access to scientific and technological resources identification CSTR

  Recently, the patent data of Chinese Academy of Sciences managed by Intellectual Property Network of China Academy of Sciences (http://www.casip.ac.cn) has been connected to CSTR (Science and Technology Resource Identification) as a whole, becoming the first patent data set connected to CSTR.
  Chinese Academy of Sciences Intellectual Property Network is a one-stop official information portal of Chinese Academy of Sciences’ intellectual property rights and achievements transformation, sponsored by the Science and Technology Promotion and Development Bureau of Chinese Academy of Sciences and undertaken by Chengdu Literature and Information Center of Chinese Academy of Sciences, and a unified information service and display platform of scientific and technological achievements in the whole hospital. At present, the website has integrated the hospital’s patents and scientific and technological achievements data, including more than 230,000 patent data, and provides information services related to intellectual property such as online patent analysis, accurate patent recommendation, patent transformation operation and online intellectual property training.
  CSTR Science and Technology Resources Identification Service Platform (https://www.cstr.cn) is built according to the national standard GB/T 32843-2016 "Science and Technology Resources Identification", which provides unique identification services for global scientific data, academic papers, scientific research institutions, researchers, scientific research instruments, patents and other scientific and technological resources. It is an important carrier for China’s national standards to serve global scientific and technological resources and a global basic service platform to promote international mutual recognition of China’s standards. CSTR has become one of the international standard protocol identifiers recognized by IANA, an Internet digital distribution organization, and has been initially applied in the Food and Agriculture Organization of the United Nations, ORCID, Harvard Dataverse, etc. Up to now, CSTR has been connected to scientific data, germplasm resources, academic papers, scientific research institutions, researchers, patents and other types of scientific and technological resources, providing multi-dimensional data analysis such as total resources, analytical volume, query volume and citation volume.
  CSTR will promote the unique identification and related discovery of patent achievements of CAS, deepen the application and service of patent information of CAS, and further improve the transformation and application level of patent achievements of CAS.
  

Can run fast and try Dongfeng popular Lingzhi M5EV long battery life version.

  [car home Original Test Drive] Friends who are familiar with the domestic MPV market must be familiar with Lingzhi, which, together with Wuling Hongguang, occupies the forefront of the sales charts all the year round. Dongfeng popular Lingzhi car has entered the MPV market for 19 years, with more than 800,000 owners. Behind its strong mass base is its product positioning of pulling people to carry goods and being economical and easy to use. The long-life version of Lingzhi M5EV based on Lingzhi M5L is also a pragmatic school. It can meet the needs of cargo logistics and short-distance connection in cities with limited licenses and restrictions, and its low charging cost is also an advantage in cities without restrictions. Today, we will take a simple test drive to experience this model.

Home of the car

  Lingzhi M5EV launched a long-life model in April this year. According to the different needs of consumers, it provides 5/7/9-seat models to choose from. This time, we experienced a top-of-the-line model.Lingzhi M5EV Long Life Edition 7-seat Luxury Model(hereinafter referred to as Lingzhi M5EV Long Life Edition),The price before subsidy is 169,900 yuan, and the price after subsidy is 144,900 yuan.The car is equipped with a battery pack with a battery capacity of 70kWh, and the cruising range announced by the NEDC Ministry of Industry and Information Technology is 420km. However, considering various working conditions and driving habits, the cruising range announced by official website is 350km.

● Design: Plain and moderate.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  The overall design style of Lingzhi M5EV long-life version is simple and moderate, and it doesn’t use the closed front face that new energy vehicles especially like now, and some popular elements are not used too much. The front grille with horizontal stripes creates a wide front vision, and the rounded body proportion gives people a simple and honest impression.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  From the side of the car to the rear of the car, the car uses more straight lines to create a calm, simple and atmospheric business atmosphere. From the perspective of external configuration, the front and rear headlights all use halogen light sources, manual folding exterior rearview mirrors and manual side sliding doors, and the rear of the car has a reversing radar and a reversing camera. The overall configuration is biased towards practical types.

● Interior and space: The second row of rotating seats has bright spots.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  As far as interior materials are concerned, the performance of the car is average. Most of the top models with a subsidy of nearly 150,000 yuan are made of hard materials, which gives people a bad feeling in terms of vision and touch. If it is used as a business reception, it is not enough.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  The 8-inch touch screen controlled by the Lingzhi M5EV long-life version is a bright spot in the interior. The pure electric MPV model at this price is a configuration that keeps pace with the times. Functionally, it has basic equipment such as navigation, mobile phone interconnection, Bluetooth phone and reversing image. For car owners who often run around to pull people out to carry goods, these functions are all practical.

  The 7-seat model of the Lingzhi M5EV long-life version adopts the layout of 2+2+3, which has many bright spots in the riding space and loading capacity, and the potential consumers who buy such models are also concerned about these two aspects. Let’s focus on the experience of these two aspects for everyone.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  Except for the lowest model, the second-row seats of Lingzhi M5EV Long Life Edition all support forward and backward sliding, and the central armrests on both sides can also adjust the angle to provide more comfortable space for the second-row passengers. On the whole, Lingzhi M5EV Long Life Edition can be regarded as conscientious regardless of the performance of the second row or the third row. However, all the above performances belong to the general operation of MPV models. The second row seat of the top model of Lingzhi M5EV long-life version also supports 180-degree rotation, and you can turn on fishing, playing cards and other modes when traveling.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  If you measure this car by the standard of short-distance connection and family trip, its performance is also remarkable. The comfort configuration in the second row and the third row is more humanized, with ventilation windows, rear air outlets and water cup holders.

● Storage capacity and test drive experience: a good hand at pulling goods.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  After talking about the daily storage space, let’s talk about its cargo carrying capacity. In order to facilitate the needs of car owners to pull goods, the third row of seats of Lingzhi M5EV long-life version also supports side hanging. If the second row of seats are slid to the front position, there will be no small space in the car, so moving and pulling goods is not a problem. However, we also remind you that this kind of side-hanging seat may cause the problem of mixed loading of passengers and goods. Occasionally, it can be used in an emergency, and it should be used with caution in other cases.

  Lingzhi M5EV Long Life Edition is driven by rear drive, equipped with a permanent magnet synchronous motor with a maximum power of 90kW(122Ps) and a peak torque of 300N·m, and matched with the motor is a single-speed fixed speed ratio gearbox.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  Lingzhi M5EV Long Life Edition has a setting, that is, after starting the vehicle, the main driver’s seat can only move forward with a seat belt fastened. To tell the truth, in our daily life, we often see drivers of such cars not wearing seat belts when driving. With this setting, they can be reminded to wear seat belts when driving to some extent.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  The car has two driving modes. For daily use, ECO mode is enough. Although the dynamic response is not very positive at the beginning, you can feel a certain driving force when you step on the accelerator pedal deeply. Needless to say, the smoothness of the motor output, the continuous and stable power makes it unnecessary to spend too much effort on overtaking in general roads in urban areas.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  For a model like Lingzhi M5EV long-life version, it is obviously not suitable for radical driving style. The adjustment of the chassis is more inclined to carry goods, and the comfort is relatively weakened. The suspension is relatively tough, and the overall rebound is not timely when passing through the speed bump. Passengers pulling in the middle and rear rows will feel redundant vibration. The overall driving texture failed to have too many surprises, which basically met its positioning.

Dongfeng Fengxing Lingzhi M5EV 2020 long-haul luxury 7-seat

  The battery pack of this vehicle has a capacity of 70kWh, and the comprehensive battery life of NEDC is 420km. The battery pack has a preheating system to avoid damage to the battery pack during low-temperature charging, and to improve the endurance and battery life of the vehicle. The battery pack is laid flat on the bottom of the vehicle, with IP67 waterproof insulation, and has passed the 300mm wading test of the whole vehicle. For private users, the car provides a warranty of 8 years or 150,000 kilometers for the power battery pack to ensure the use of the car.

Full text summary:

  Lingzhi M5EV is an attempt made by Dongfeng in the field of electric MPV. Although it is improved on the basis of fuel version, it is not mature enough in many places, but one of its major advantages is that there are fewer models of this type on the market at present. For small business owners in cities with limited purchases and restrictions, such an unlimited, practical and low-cost model is perfect. The cruising range of 420km and its flexible second and third rows of internal space are also good partners for daily life and weekend leisure. If you need a practical car, and you don’t have high expectations for configuration and power driving, you can take a look at the Lingzhi M5EV long-life version. (Text/photo/photo car home Jiang Tianshuang assisted Yao Jia)

9.3 points! The welfare returns in the middle of the night, and I look silly …

I finally got off work-

You look at the crowded subway, sigh and stick it up. When you get home, you can unload your fatigue, open a hit drama and enjoy the rare free time.

This is the spiritual comfort of contemporary migrant workers-binge-watching.

But these spiritual foods are often uneven, especially seasonal dramas, which can be called unfinished professional households:

One season reached its peak, the level declined in two seasons, and the three seasons were beyond recognition …

Is there a season drama that breaks the curse of the rotten end?

There is!

Ten years, it’s as usual-

When the ninth season started, it still got a high score of 9.3. What’s the secret?

NO, "The Lonely Gourmet" not only doesn’t have a secret book to keep it hot, but even the story is very simple. It has been telling the same thing for nine seasons-

One is calledKogoro, head of the wellA grocer (decorated by YUTAKA MATSUSHIGE) looks for delicious food during work breaks.

The story of Goro and food has also become the youth of many people, and every season, some people are willing to come again for hundreds of millions of times.

At the beginning of the ninth season, Wu Shu, who has not seen for a long time, stood at the door of the food store, and his memories popped up instantly.

"Knocked-"

Uncle Wu, who just finished the negotiation, is hungry.

This familiar picture, my DNA also moved …

Uncle Wu found a small shop that fried pork chops, and watched the diners in the shop feasting. Uncle Wu’s greedy worm was completely hooked up.

Not much to say, let’s have one firstFried pork chop with loin meat——

The crispy shell is wrapped in thick pork chops, topped with sauce, and the meat flavor and sauce blend perfectly, which is the top enjoyment of taste buds.

Second mealGolden fishWonderful, the soft fish fully absorbs the sauce, picks up a piece and chews it carefully, and the fresh fragrance of the fish rolls in the mouth, absolutely!

Not only can you learn about Japanese food with Uncle Wu, but you can also see Uncle Wu occasionally.Tasting European food.

This time, Uncle Wu brought us here.Greece.

Greek cuisine is not on the same channel as East Asia in terms of material selection, and it is full of strong European flavor.

Can you imagine that grape leaves can also be made into food? !

Uncle Wu still played his gift of eating ~

Pour lemon juice, and the acidity will be enhanced, which is another flavor.

In addition to foreign food, The Lonely Gourmet also has an essential link-Chinese cooking.

This time, Uncle Wu ateGuizhou cuisineGuizhou cuisine is spicy and delicious, which is quite different from that in Sichuan and Chongqing.

This dish of Sichuan style pork is rich in color and fragrance, and the fat but not greasy meat slices are mixed with the fragrance brought by pepper, which is spicy and cool!

How can there be no lobster sauce hot pot in Guizhou restaurant?

Amorphophallus konjac, baby cabbage, Flammulina velutipes, potato chips, thousand pages of tofu …

There is also a hot pot standard-mutton.

The steaming picture is matched with Wu Shu’s inner monologue, and the smell of hot pot seems to have overflowed the screen ~

Uncle Wu’s monologue when eating hot pot is a deep understanding of hot pot.

The spicy and refreshing feeling stimulates the taste buds, and even if you sweat, you won’t give up the delicious taste. This feeling can be accurately described only by "enter the dragon", which is not bad.

The subtle metaphor makes the food come alive instantly.Every psychological activity is a prelude to the strategy of food, and it is the pure attitude towards food that makes The Lonely Gourmet become a drama for many people.

And this thing, once done, is ten years.

"Lonely Gourmet" was launched in 2012, and it has been ten years since then. Uncle Wu has also moved from middle age to old age.

This foodie uncle who is rich in inner drama and likes to gossip is the youth of many people. Seeing him eating with relish will ease his gloomy mood.

We are used to "I’m so hungry!" after Uncle Wu’s discussion. ;

I also know that after he enters the store, he will make a choice phobia in the face of dazzling food;

When enjoying food, he can always find an accurate description of the angle and let the audience be there.

It seems that everything is used to it, but in fact it can’t be separated from the actors’ efforts.

YUTAKA MATSUSHIGE won’t eat for a whole day before each shooting, and only in this way will he feel ecstatic when shooting.

This season’s filming is particularly difficult. During the epidemic, YUTAKA MATSUSHIGE wore a mask and shuttled through the food store, and also spoke frankly about the impact of the epidemic on the catering industry.

In addition, there is no ups and downs in the whole drama, and no characters have cooperated with YUTAKA MATSUSHIGE for a long time. The drama is supported by the strength of the actors.

In the play, Uncle Wu is looking for food alone. Off-stage, it was YUTAKA MATSUSHIGE who made a one-man play for ten years.

Uncle Wu and YUTAKA MATSUSHIGE have a certain degree of overlap, and at some point, they are all lonely.

Although the characters and plot of Lonely Gourmet are slightly thin and the shooting environment of the ninth season is not ideal, itStill holding the purity of food, coming to us with a cure..

The Lonely Gourmet lasted for many years and gained a large number of loyal audiences.

There is nothing but-healing in resonance.

Uncle Wu is actually a microcosm of modern people.

The people behind him are more in the cubicle, in the assembly line and on the way to work.

Modern life divides everyone into independent individuals, and the more people travel alone in the stream of people.

Sometimes it seems like calling friends, but the deep loneliness is lingering, which is a common fault of modern people.

Lonely is not a gourmet, but you and I who hurried home after the lights went out.

The reality is a little sad, but the appearance of Uncle Wu saved the sorrow.

"Lonely Gourmet" perfectly hides the tired side of Uncle Wu under the delicious food, seemingly enjoying the delicious food, but actually curing loneliness.

In the only free time, I throw away all my troubles and eat without distractions, which not only makes my stomach full but also decompresses.

just asThe beginning of each episode——

The pictures in this article are from the Internet. If there is any infringement, please contact and delete.


Being separated from the world during food time, gaining temporary spiritual freedom and eating well are the lowest cost sources of happiness.

So, did you have a good meal today?

The commander of the Israeli navy is of China descent and has achieved extraordinary things.

  


  Israel’s new naval commander, Eli Marom


  The "first" in the army


  At the end of October, Israel’s new naval commander, Aili Maroum, officially took office. Unlike his predecessor, as well as officers of all arms such as the Israeli navy, Ali has a distinctive China silhouette, and his face, skin color and hair are quite "China-like", so that he looks like a China at first glance. It turns out that Ellie is of China descent. It is the first time in Israeli history that a person of China descent holds such a high military post.


  On October 8th, Ali Maroum was officially appointed. On the same day, Israeli Defense Minister Barak, Israeli Chief of Staff Askin Naz and others held an appointment ceremony for him. Barak admired Ali and had high hopes, because he was interested in David, the former naval commander. Ben? Basat’s performance in the Israel-Lebanon war was "extremely dissatisfied", and he always wanted to "take off" the admiral who had worked for four years, but he was constrained by the relations between all parties and did not do it easily. This time, Barak finally made a decision. He patted Ali on the shoulder and said, "Work hard, make up for the losses caused before, and rebuild the elegance and glory of the Israeli navy." Askin Naz also encouraged Ali: "It’s up to you now!"


  "Alternative" makes extraordinary achievements.


  The 52-year-old Aili’s grandfather is a native of China, and her grandmother moved to China from Russia. Aili’s father fled from Germany to China in World War II, met Aili’s mother and got married. Seven years after the establishment of Israel, the couple returned to Israel to settle down, and Ali was born here.


  Because Ellie’s oriental characteristics are obvious, she has been called "Chinese" since she was a child. This nickname comes from the English word "Chinese" (from China). From the time he was sensible, Ali realized that his appearance was "different", but he didn’t resent being called "Chiney". Even when introducing himself to strangers, he took the initiative to say "My name is Chiney". According to Ali’s classmates, he was always at the top of his class and had a strong personality. After joining the army, Ali’s strong personality became more prominent, which made him get promoted continuously. One of Aili’s colleagues said: "The fact that he looks different forced Chiney to constantly show people that he is better, so that he quickly became one of the best."


  Through hard work, Ali became the commander of Haifa naval base in northern Israel from a little-known naval engineer, and became the deputy commander of the navy. In addition, he has served as the Israeli military representative of the United States-Israel Joint Staff and the Israeli military attache in the United States.


  Be questioned for no reason because of one’s ancestry


  After Ali was appointed as the commander of the navy, it caused great repercussions in Israel’s domestic public opinion and people. On the same day, the Israeli media reported the incident with headlines such as "Israel has an China as the commander of the navy" and "the new commander of the navy comes from China", which was quite confusing, and some netizens who didn’t understand the situation even gave a puzzled exclamation. Many public opinions have expressed enough confidence in Ali’s ability to reorganize and strengthen the Israeli navy, but some people have questioned his appointment as commander of the Israeli navy, and some even point out that he is a quarter of China origin. (Global Times Global Network)


  


  


  Israeli navy "Saar-5" light frigate [data picture]


  


  


  Delivery ceremony of Israeli "Dolphin" class conventional submarine [data picture]


  Little information: Israeli navy


  Due to the persistent tense military and political situation in the Middle East, Israel has always attached great importance to the navy and developed it as an independent service for many years. By the beginning of 2003, the Israeli navy had a total of 65,000 people and more than 70 ships including submarines, making it an important maritime force along the Mediterranean coast.


  Organization


  The highest officer of the Israeli navy is the commander of the navy. Through his headquarters (located in Haifa), he exercises daily leadership over the naval forces: he is responsible for the naval combat readiness, development, organization of combat training and material and technical support, and is directly under the leadership of the Chief of Staff of the Israeli Armed Forces. The headquarters consists of six departments: combat department, training department, reconnaissance department, cadre department, communication department and logistics department. The organizational structure of the navy also includes several headquarters, namely, the naval combat force command, the missile force command, the submarine force command, the patrol force command, the coast guard command, the naval base and the naval berth command. ·


  All the ships of the Israeli navy are organized into two clusters: the Mediterranean cluster and the Red Sea cluster. The submarine sub-fleet, the light frigate sub-fleet and the missile boat sub-fleet are the main naval fleet.


  By the beginning of 2003, the Israeli navy’s combat formation consisted of four sub-fleets of combat ships (one submarine sub-fleet, one light frigate sub-fleet and two missile boats sub-fleet), six combat boat detachments (five escort boats and one missile boat and escort boat detachment), one base patrol squadron, two helicopter battalions, two independent naval battalions (Marine Battalion and Destruction Reconnaissance Battalion) and 12 coastal battery companies. At present, there are three Israeli naval bases, namely Haifa base, Ashdod base and Eilat base. In addition, it has several berths, such as Aka, Art Park Jung Su and Ashkelon. Except Haifa base, other ground facilities are located in the Mediterranean Sea.


  Existing equipment


  According to the latest information, the Israeli navy currently has six conventional submarines of Dolphin and Gaelic class, three light frigates (Saar-5 class), 20 missile boats (Saar-4 class and Saar-4.5 class) and 33 escort boats (Dabbour class, Super Debora class and Sheldag class). As an underwater killer of the Israeli navy, the Dolphin class submarine is equipped with 533mm and 650mm anti-submarine torpedo tubes and UGM—84C Saber-Harpoon anti-ship missile. According to western military analysts, the boat is also equipped with cruise missiles developed by Israel itself.


  In addition, the naval air force is equipped with three base patrol aircraft and 41 combat helicopters. The coastal artillery has 24 shore guns and 60 anti-aircraft guns with a caliber of more than 20 mm.


  In the event of war, the number of sub-fleets and formations of the Israeli navy will not change at the beginning, but the number of warships and combat boats can be increased by 21 after 30 days and nights. With various mobilization measures, the total number of Israeli navy will reach 115,000.


  Personnel training


  Israeli naval officers are usually trained by the Naval School in Haifa, which is attended by active naval sergeants and those who have successfully graduated from the maritime school in Akka. The training period is two years, and graduates will be awarded the rank of lieutenant. About 50 officers graduate from this school every year.


  The Naval Training Center in Haifa is also a place to train command cadres and engineering and technical cadres for the navy, mainly to train junior technicians working on ships. In addition, graduates from the merchant marine school in Tyre-Avev and Ashdod can also be enlisted in the navy.


  The training of middle-level officers in command and command positions is usually carried out in their own command colleges and military academies in NATO countries.


  Its campaign tactical training is carried out according to the plan of the General Staff of the Israeli Armed Forces. The main purpose of training is to teach and improve the skills of organizing the coordinated action of various arms of the navy. One of its contents is the "Reliable Mermaid" exercise jointly conducted with the US and Turkish navies. The exercise is held regularly in the eastern Mediterranean, with the aim of constantly improving the coordinated actions of the three navies in the process of coordinated search and rescue operations. When organizing the training of staff officers, they widely use computer equipment, including equipment that can simulate the combat operations of all parties and equipment that can improve the training level of commanders with reduced military input.


  Future development


  Within the framework of the "Army-2010" package to re-equip the national armed forces, Israeli leaders paid special attention to the modernization of the navy. The improvement of naval forces is carried out from the following aspects: new surface ships and submarines are added to the navy, equipped with modern weapons and technical weapons, and the modernization of various ships is realized. According to this plan, Israel will build six "Super-Debora" class escort boats and two "Sheldag" class escort boats. Among them, the first boat was put into service in the second half of 2003. Moreover, Israel’s Ministry of National Defense plans to buy another seven similar escort boats.


  At the same time, the Israeli navy is negotiating with the American shipbuilding company "ingels" for the purchase of four light frigates. If the negotiations go smoothly, the first batch of ships will be installed as navy in 2007. According to the analysis of international military analysts, it is very likely that the Israeli navy will be equipped with cruise missiles with a flight distance of about 200 kilometers. The missile was developed by a subsidiary of the Israeli aviation industry giant Konzern.


  According to the estimation of international military analysts, the new naval plan will further improve the combat capability of the Israeli navy and consolidate its dominant position in the navies of Arab Muslim countries in the Near and Middle East.

Editor: Cao Jin

The annual meeting can’t stop! The release of the key was postponed until February 28th.

1905 movie network news The movie key, which is directed by the screenwriter and starring,, and will be extended to February 28. As of 22:00 on January 18th, the cumulative box office of the film was 844 million yuan, winning the single-day box office champion for 18 consecutive days.


The annual meeting can’t stop! It tells the story that fitter Hu Jianlin was transferred to the group headquarters by mistake and became a gold collar. At the annual meeting of the group, he exposed the corrupt executives of the factory and finally saved the factory. The film’s joyful irony of the strange phenomenon in the workplace has deeply resonated with countless migrant workers, helping them to climb their reputation. Douban scored as high as 8.2, making it the highest domestic comedy in Douban in the past five years.

Tesla China responded to the rumor that FSD autopilot entered China: There is no accurate timetable at present.

On February 28th, according to Sina Technology, the customer service of Tesla China responded to the rumor that FSD is fully autonomous driving and will soon enter China. At present, there is no exact time for the launch of FSD in China, so we can keep an eye on it.

According to the feedback submitted by the IT House friends, a blogger "Tesla China Analyst" posted on Twitter this afternoon that FSD will enter China within two weeks and released a photo of the car-machine interface.

This is not the first time this year that Tesla FSD has entered China. In January of this year, the news that Tesla FSD version 12 will be pushed in China at 3 am on January 8, 2024 Beijing time appeared on the Internet. Some insiders of Tesla China responded: "" This rumor also said that "this version will be fully launched in China for the first time", and it also brought "fine adjustment of the driver’s seat door angle" and other contents.

However, as early as last November, with, Tesla FSD was also considered to enter the "countdown" stage. According to the report of China Fund at that time, Tesla China once responded: "".

On the 10th of this month, the news source @Teslascope monitored that Tesla has started to push the update of 2023.44.30.15 to its employees and special groups, including the FSD Beta 12.2 version of the assisted driving function. Tesla’s last FSD Beta version was V12.1.2, which was only open to employees and about 100 public beta users. These 100 people are probably the "special groups" mentioned by Tesla.

Related reading:

FSD Beta 12.2 has been opened to Tesla employees and special groups.

"Tesla China insiders responded to" Pushing FSD 12 Autopilot in China on January 8 ":False News"

Ten questions and ten answers about stem cells, and I will learn about her magic with you.

Through ten groups of questions and answers, this paper gives you the function and application of popular science stem cells, and shows you the important position of stem cell therapy in modern medical care.

Mother, the pilot of the boat of life, not only gave birth to us, but also promoted the reproduction of mankind. She is a great inheritor of life and a transmitter of love.

Cells, the weavers of the web of life, constitute the cornerstone of the human body, which is a miracle exhibitor with various forms and complicated functions.

The human body is a magnificent movement interwoven with countless cells, the harmonious resonance of 240 kinds and 60 trillion cells, and the subtle fusion of 4 major organizations, 36 organs and 8 major systems.

She is a special cell, but also the guardian of the source of life. She can regenerate new cells, replace aging cells and repair damaged tissues and organs. She is the mother of the cell world.

This kind of cell is the root of our life-stem cells.

With the progress of science, stem cell research has become one of the most advanced and active research directions in the global medical field.

However, for the general public, what exactly are stem cells? What does it do? What kind of people are suitable for repair intervention through stem cells? What are the risks even in treatment? This article will help you understand these questions easily through ten groups of questions and answers.

Ten questions and answers about stem cells

01

What are stem cells?

Stem cells are cells with self-renewal function and multi-directional differentiation ability.

Under certain conditions, these cells can differentiate into more than 220 different functional cells in human body. Because of its remarkable homing effect, stem cells can repair and replace damaged cells and promote organ regeneration. They are the basic cells that constitute various tissues and organs of the human body, so they are known as "the source of life" and are often called "universal cells" in the medical field.

02

What are the classifications of stem cells?

Scientific research shows that stem cells exist not only in embryos, but also in adults, which can be extracted from cord blood, umbilical cord, placenta, bone marrow, fat and other tissues.

Stem cells are divided into totipotent stem cells, pluripotent stem cells and unipotent stem cells according to their extensive differentiation ability.

Stem cells can be divided into embryonic stem cells and adult stem cells according to their stages of embryonic development.

According to the tissue type of stem cell differentiation, it can be divided into hematopoietic stem cells and mesenchymal stem cells.

03

What is stem cell therapy?

"Stem cell therapy" refers to a medical intervention that extracts stem cells from patients or uses other stem cells, cultivates them under laboratory conditions, and then transfuses them into patients through specific transplantation techniques, so as to repair the cells and tissues that have lost their functions, thus promoting their regeneration and improving their symptoms. At present, hematopoietic stem cells and mesenchymal stem cells are widely used in clinic.

04

What is the difference between autologous and allogeneic stem cells?

Autologous stem cells are derived from patients themselves, which have perfect tissue compatibility, no need for matching, high utilization rate of transplanted cells and no rejection.

Allogeneic stem cells are derived from individuals other than patients and have low immunogenicity. Autologous stem cells are not recommended for people with infectious diseases, major genetic diseases or other health problems.

05

How can stem cells be used to treat diseases?

Cells are the basic unit of life. Human life is accompanied by the process of self-replication and differentiation of stem cells from birth to growth. The ability of stem cells to treat diseases mainly stems from their biological characteristics, which include:

The ability of long-term survival and continuous self-replication;

It can differentiate across different lines and germ layers, showing high plasticity;

And its chemical tendency, that is, homing effect, provides an important biological basis for cell therapy.

06

Is stem cell therapy risky?

Compared with conventional drug therapy, stem cell therapy is considered safer and has fewer side effects. But this does not mean that the risk is zero. Looking at stem cell therapy objectively, the following are some main risks of stem cell therapy:

Tumor development: If stem cells divide or differentiate into abnormal cells uncontrollably, it may lead to cancer. This mainly applies to embryonic or fetal stem cells, or reprogrammed stem cells, because the risk is based on their pluripotency.

Immune rejection: The body may regard foreign stem cells as a threat and attack them, leading to inflammation and injury. This is more likely to happen in hematopoietic stem cells (HSC).

Infection or complications related to invasive medical procedures: Stem cell therapy may lead to infection or complications related to invasive medical procedures.

Drug interaction: Stem cell therapy may have unpredictable interactions with other drugs taken by patients, which may lead to adverse drug interactions.

Unconfirmed or untested stem cell therapy: There is also the risk that unscrupulous practitioners provide unconfirmed or untested stem cell therapy, which may be unsafe or effective.

The effect of treatment is uncertain: patients should also realize that stem cell therapy does not guarantee a cure, and in some cases, it may not be effective at all. Stem cell therapy may not provide lasting results, and patients may need additional treatment.

It should be noted that these risks may be different due to individual differences, treatment methods, stem cell types and other factors. Before receiving stem cell therapy, patients should fully discuss these risks with doctors and weigh the potential benefits and risks of treatment.

07

What diseases can stem cell therapy treat?

Stem cell therapy is mainly used in the fields of treatment, health care and beauty, and it is also used to improve some refractory diseases.

Heart disease: Stem cells can regenerate damaged heart cells and maintain the health and vitality of the heart through a specific process of induced differentiation in the treatment of heart disease.

Prevention and treatment of Alzheimer’s disease: Injecting induced differentiated stem cells can replace aging brain cells and improve cognitive function.

Treatment of liver cirrhosis: generate liver cells through differentiation, and promote the regeneration and repair of liver cells.

Cancer treatment: Directionally differentiated stem cells can enhance the body’s immune function and help fight tumors.

Treatment of premature ovarian failure: Stem cell therapy can restore ovarian function, promote the repair of damaged tissues, improve ovarian microenvironment, enhance reproductive capacity, prevent follicular atresia and promote follicular renewal.

Male hypofunction: Stem cell therapy can repair the aging of reproductive system, improve sexual function, maintain the balance of sex hormones, reduce nocturia, relieve the symptoms of prostatic hyperplasia, and help prevent prostate hypertrophy and other problems.

Enhance muscle function: with the increase of age, muscle function weakens, strength and relaxation function decline, and the body is bloated. Stem cell therapy can improve muscle function, enhance strength, improve body shape and show youthfulness.

08

What is the effect of stem cell therapy?

Stem cell therapy can be carried out by systemic infusion or fixed-point intervention. Clinicians adopt appropriate cell types and treatment strategies according to patients’ health status and disease needs.

Because this treatment can directly intervene in the cause of disease, avoid other physical injuries that may be caused by drugs and surgery, and help regulate the sub-health state, the effect is usually not limited to treating diseases, but also improving the overall function.

09

Is there an age limit for stem cell transfusion?

Traditionally, some types of stem cell transplantation, such as allogeneic bone marrow transplantation, often have age restrictions, and it is usually recommended to be 50 years old or under 45 years old. However, with the progress of medical technology, this limitation is being broken.

For example, it has been reported that a 71-year-old patient successfully received autologous hematopoietic stem cell transplantation, and a 69-year-old woman also successfully received her son’s hematopoietic stem cell transplantation.

These cases show that under proper medical conditions and monitoring, elderly patients may also successfully receive stem cell transfusion therapy, which proves that the age limit is gradually relaxing.

10

What are the taboo populations of stem cells?

Patients with advanced malignant brain tumors, especially brain tumors;

Patients with shock or general failure with abnormal vital signs (requiring disease correction) and those who do not cooperate with the examination;

Patients with heart, lung, liver, kidney and other important organ dysfunction (multiple organ dysfunction syndrome);

Patients with dysfunction of coagulation mechanism;

Patients with severe local infection;

Patients with positive serological results (such as HIV, hepatitis B, syphilis, etc.);

People with high allergic constitution or severe allergic history;

People with nervous system diseases or diseases that have not been diagnosed;

This group is in a special situation and has uncontrollable risks, so it needs careful diagnosis and treatment.

Write it at the end

Stem cells have excellent regeneration and repair functions, and these abilities will gradually weaken with age. Therefore, it is an increasingly popular choice to store healthy stem cells as early as possible, which will provide the possibility for future health management and disease treatment.

The 21st century will be the era of cell therapy, and the application of stem cells and immune cells is expected to play an important role in clinical treatment.

However, at present, except for the mature clinical application of hematopoietic stem cell transplantation, other types of stem cell therapy are mostly in the research stage, and strictly designed clinical trials are needed to prove their clinical advantages, safety and effectiveness, which need further study.

Moreover, non-compliant and eager stem cell therapy and research not only threaten the safety of patients, but also hinder the improvement of stem cell therapy and its research level, which needs the attention of legal supervision, and patients with hope in stem cell therapy should also be alert to fraud and pay attention to its development and related policies and regulations.

Original title: "Why do you call her" the mother of cells ":Ten questions and ten answers about stem cells, and learn about her magic with you"

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Some dating software is still "undefended" for minors.

  Multiple social apps can still be used normally without "real-name authentication". Insiders suggest that the youth mode should be turned on by default.

  Recently, many netizens found and reported that some dating software is not strict with the use of minors. The Beijing Youth Daily reporter found that when using multiple strangers’ dating software, there is no need for real-name authentication, and minors can use these strangers’ social software "unimpeded". It is particularly noteworthy that in terms of function, there is no difference between unauthenticated accounts and other accounts, and accounts without real-name authentication can also purchase adult products at will. In this regard, many people in the industry have suggested that the youth mode can be turned on by default when the user does not log in with an ID card.

  find

  Multiple social App users can also be rewarded without real-name authentication.

  The reporter of Beiqing Daily recently found that many social apps, such as soul, Detective, Momo, etc., can be used normally by simply choosing to log in with WeChat or mobile phone number without "real-name authentication".

  These platforms all suggest that users must be over 18 years old when registering. For example, after soul registers, users need to choose the date of birth, but users can enter the interface without hindrance by filling in a date over 18 years old at will. Among them, soul needs to use a virtual avatar, and exploration and Momo need to add a real and clear avatar.

  In the process of use, the above App will send a pop-up window in teenager mode to the user. The reporter of Beiqing Daily noticed that the youth mode entering Soul needs real-name authentication, and people’s faces will be dynamically recognized during the authentication process. After the tester enters his real name information, he can’t enter the teenager mode again. The teenage mode of exploring and momo is to set the password directly.

  In view of the fact that it can be used normally without real-name authentication, Momo customer service responded: "Some functions need real-name use." When asked which functions will be affected by not having a real name, the customer service did not give a positive answer. In addition, regarding whether the real name will restrict consumption, the customer service said: "No real name will not restrict consumption."

  When using the live reward function of Detective and Momo, even the account without real-name authentication will automatically jump to WeChat or Alipay.

  In terms of recharge, none of the above three softwares has relevant restrictions or secondary verification for minors. And there is no real name account and unlimited consumption, which can recharge the highest amount of soul coins, exploration coins, momo coins and VIP recharge.

  experience

  Push content is undifferentiated, making friends and shopping are not affected.

  The reporter of Beiqing Daily used an account without real-name authentication on a dating software, and marked "the second day" in the introduction. After searching for keywords such as "making friends in junior high school", "sexy underwear" and "adult products" on the home page, a lot of related push content appeared. The purchase of goods can automatically jump to WeChat or Alipay. In other words, if minors are not authenticated in this platform, it will not affect their use.

  After several keyword searches, Beiqing Daily reporter used the test account to open the above-mentioned dating software again, and the homepage push also became the corresponding content related to keywords, in which minor dating also appeared directly and many times. In the shopping column, most of the products pushed also meet the previous keywords.

  In the actual measurement, the reporter of Beiqing Daily also found that there are multiple video platforms in the youth protection mode, and the platforms will impose restrictions on young users in terms of content, usage duration and consumption.

  For example, the daily use time should not exceed 40 minutes, and it is forbidden to use the software from the evening to the next morning, and it is impossible to open or watch the live broadcast, reward, recharge, cash withdrawal and so on. For example, a survey shows that in the youth mode, except for the functions of mobile phone recharge and city service, other functions of life service, transportation and shopping consumption are all disabled, while the functions of financial management are directly hidden. Aauto Quicker and Tik Tok, short video applications, banned teenagers from watching live broadcasts, which fundamentally prevented the risk of teenagers’ recharge and reward. Tik Tok also closed Tik Tok Mall. Video platforms Iqiyi, Tencent, Youku and Mango TV have closed the membership center and all other consumption channels such as financial welfare and games. Bilibili has also closed consumer operations such as membership purchase and top-up rewards.

  suggestion

  Directly open the youth mode for the unauthenticated netizen platform.

  In response to the measured results of the reporter of Beiqing Daily, Professor Shang Jianhui, deputy dean of the School of Journalism and Communication of Hebei University, believes that the platform practice that has not yet imposed restrictions on young users does not conform to the norms. "According to the explicit requirements of the Regulations on the Protection of Minors’ Network (Draft for Comment) and the Regulations on the Protection of Children’s Personal Information Network, the network platform should formulate special rules and user agreements for the protection of children’s personal information. Therefore, this practice of the platform should be improved, otherwise it may cause harm such as premature maturity of children and deception of the network. "

  Professor Shang suggested how to improve the platform’s protection measures for minors. "At present, there are some voices in academic circles. The index system of minors’ network protection includes 19 indicators in six dimensions, including information prompt, technical protection, anti-addiction (comprehensive) management, emergency complaint and reporting mechanism, privacy and personal information protection system, scientific popularization and publicity and education."

  "Technical protection, information tips, scientific popularization and publicity and education are the weak links in the protection of minors on the Internet platform. The platform should first strengthen technical security protection and realize standardized management of the platform; Secondly, construct an omni-directional information prompt matrix to optimize the use environment of minors; Furthermore, practice the concept of multi-knowledge inclusiveness and do a good job in popular science and publicity and education; Finally, improve the youth model and enrich the content pool for minors. " Professor Shang said.

  Some operators who did not want to be named told the reporter of Beiqing Daily that "registering with a mobile phone number does not mean real-name authentication. Operators will not synchronize all users’ personal information to all platforms at will. It is not excluded that operators cooperate with some larger platforms to authorize the platforms to identify information through mobile phone numbers, but only if both parties sign an agreement to ensure the other party’s safe storage and standardized use of personal information. Therefore, it cannot be said that registering with a real-name mobile phone number is equivalent to real-name authentication. "

  Earlier, Jiang Yaodong, member of Chinese People’s Political Consultative Conference and former vice president of China University of Mining and Technology (Beijing), also suggested that "for users who have not been authenticated, the platform can default the user to enter the youth mode, which can protect minors from browsing age-appropriate content to the greatest extent."

  interlinkage

  According to public data, in 2021, the number of underage netizens in China reached 191 million, and the Internet penetration rate of minors reached 96.8%. According to a survey released by Beijing Internet Court, from June 2022 to May 2023, Beijing Internet Court accepted a total of 143 civil disputes involving minors, and the number of cases increased year by year, with game recharge and live rewards accounting for the largest proportion. Among them, the phenomenon that minors involved in litigation evade family supervision and anti-addiction measures is more prominent, and most minors evade family supervision and platform authentication measures. At the same time, minors are easily influenced by bad information. Some minors use the Internet to browse pornographic, bloody and violent bad content, and some minors are influenced by the online war and the "network water army" and learn to imitate bad online behavior.

  Text/reporter Wen Wei Intern Li Yujie

Regenerative Medicine Breakthrough China Scientists Induce Human totipotent Stem Cells.

  Recently, researchers from China Academy of Sciences, Shenzhen Huada Institute of Life Sciences and other institutions have cultivated human pluripotent stem cells in a state similar to that of fertilized eggs developing for three days through somatic cell induction. This is the "youngest" human cell cultured in vitro in the world at present, and it is another subversive breakthrough in the field of regenerative medicine after scientists successfully induced human pluripotent stem cells. Related research results were published in the international academic journal Nature in the early morning of March 22nd, Beijing time.

  It is understood that researchers have developed a non-transgenic, fast and controllable "cocktail" cell reprogramming method, which can transform human pluripotent stem cells into totipotent 8-cell embryonic-like cells, that is, totipotent stem cells equivalent to the three-day development of fertilized eggs. This achievement will help to realize the in vitro regeneration of human organs in the future, and it is of great significance to solve the problems of organ shortage, allograft and xenotransplantation rejection.

  In 2012, the Nobel Prize in Physiology or Medicine was awarded to shinya yamanaka, a Japanese scientist who successfully induced mature somatic cells into pluripotent stem cells in blastocyst stage. The cells in human blastocyst stage are fertilized eggs developing 5-mdash; In the state of 6 days, its ability to further develop is limited.

  However, this study has made a big step forward in this field, and for the first time, embryonic cells with fertilized eggs divided for only 3 days were obtained. In the early stage of fertilized egg development, great changes have taken place every day, and it is these 2-mdash; In 3 days, scientists obtained human 8-cell embryonic-like totipotent stem cells through in vitro induction for the first time. This is the "youngest" human cell induced in vitro so far, and it has very strong development potential. This study will also help to unlock the key to the early development of human embryos.

  According to reports, these totipotent 8-cell embryonic-like cells reconstructed the embryonic state of fertilized eggs after only three times of division. Compared with pluripotent stem cells in the past, these cells can differentiate into placental tissues and may develop into more mature body tissues.

  "This progress is also a model of the combination of regenerative medicine and single cell sequencing technology." Dr. Liu Longqi from Shenzhen Huada Institute of Life Sciences, one of the authors of the paper, said, "The efficient identification and mechanism analysis of cells or tissues obtained by stem cell technology in vitro or in vivo will greatly accelerate the development of regenerative medicine."

  This is the first time that researchers have "transformed" human pluripotent stem cells into pluripotent embryonic cells in a real sense, so that people can reverse the "adult" version of cells into "infant" versions with more possibilities. At the same time, because the totipotent cells obtained this time are closer to the original state of early embryos, if they are used in regenerative medicine, the cultivated organs will be closer to the state of real organs, which is more conducive to transplantation.

  This breakthrough is due to the progress of single cell sequencing technology. In the past, researchers may have to process and culture thousands of cells, and the probability of success is less than 10%. Nowadays, based on the single-cell library sequencing platform (DNBelab C4) independently developed by Huada University, combined with the DNBSEQ sequencing technology created by Huada Zhizhi, scientists can conduct multidimensional single-cell analysis with high sensitivity and accuracy, quickly obtain cells with important development potential, and study the development direction of these cells.

  In this study, the research team also classified the induced pluripotent stem cells and injected them into mice for further development, and then used the single cell sequencing technology of Huada to conduct large-scale cell map analysis. Finally, the researchers confirmed that the experimental totipotent stem cells were highly similar to human 8-cell embryonic cells, which proved the totipotency of the cells. This provides a scientific basis for organ culture using patients’ own cells in the future and for organ transplantation and replacement.

  The research was led by China Academy of Sciences and Shenzhen Huada Institute of Life Sciences, with the participation of several research teams including Cambridge University, Jilin University and rajshahi University. This study has passed the ethical review and strictly followed the corresponding laws and regulations and ethical standards.